Journal: Bioengineering
Article Title: Highly Porous Type II Collagen-Containing Scaffolds for Enhanced Cartilage Repair with Reduced Hypertrophic Cartilage Formation
doi: 10.3390/bioengineering9060232
Figure Lengend Snippet: The incorporation of CII was associated with a reduction of COLX synthesis, and in combination with HyA, was found to increase the accumulation of COLII in the matrices by day 28. The expression of COLII ( A ), COLX ( B ) and GAPDH (housekeeper) ( C ) proteins was determined for scaffolds seeded with human MSCs from donors 1, donor 2 and donor 3 after 28 days in culture. COLII and COLX protein expression was also determined on cell-free scaffolds ( D ). The relative protein-band intensity of COLII and COLX was quantified and normalised to GAPDH ( E ).
Article Snippet: The membrane was incubated at 4 °C overnight with a 1:1000 solution of diluted rabbit monoclonal [EPR12268] anti-type II collagen (COLII) antibody (Abcam, ab188570), 1:1000 diluted rabbit polyclonal anti-type X collagen (COLX) antibody (Abcam, ab58632) and 1:5000 rabbit polyclonal anti-GAPDH (GAPDH) antibody (Abcam, ab9485).
Techniques: Expressing